Benefits

Inoculation either with Green Quality Max or with the PGPR line enhances your cultivations through the following mechanisms:

When you inoculate your seeds either with our Inoculants or our Biofertilizers, the synergism of some or all of these benefits allows you to obtain a very important global effect that results finally in higher yields for your cultivation.

Features of Our Products

Bacteria: PGPR Wheat and Barley, PGPR Corn and Sorghum and PGPR Sunflower products, have the benefits of the best known growth promoting bacteria: Azospirillum brasilense and Pseudomonas fluorescens are combined in a single product.
In PGPR Soya, the Bradyrhizobium japonicum bacterium, which is indispensable for this cultivation and forms nitrogen fixing nodules, is also included. Therefore we obtain a tri-bacterial product which improves the yield of your Soya cultivation systematically.
In the case of the Inoculant for Soya, Green Quality Max, only stocks of Bradyrhizobium japonicum in a concentrated form and of easy application are included in the formulation.

Selected Stocks: So as to improve the performance in your production we have differenced our product looking for the combination of the most effective stocks for every cultivation, this allows us, together with adjustments in quantity, bacteria proportion and formulation, to obtain a high performance product which is specific for the most important cultivations in the country.

Peat: In our products, bacteria come together with a revolutionary formulation that combines the best of the liquid cultivation and the solid cultivation thanks to the incorporation of peat. Peat acts as an organic base that lodges bacteria and allows them an efficient protection and survival, improving bacterial recounts remarkably and allowing to obtain the product with the highest stability in the market.

Incorporated Protector: To improve the performance of Inoculants and Biofertilizers in your cultivations, we have also been the first ones to incorporate in our formulation the adequate protector which improves bacterial survival in your seeds considerably to facilitate, in this way, the application of the product and allow better performance in the Inoculation or Biofertilization. The protector complements the peat’s effect and avoids the hard steps of pre-mixing required by the rest of the market products, since it is applied directly on the seed.
In the case of the Green Quality Max Inoculant, we have achieved such an accurate formulation that the pre inoculation can be performed up to 7 days before the sowing.

Micronutrients: Bacteria’s performance in the rhizosphere (soil portion that surrounds and contains the roots’ surface) depends upon the right supply of micronutrients, since the lack of these lesser nutrients diminishes the competence of the added bacteria with relation to the soil’s natural bacteria. Besides, the supply of micronutrients enhances seedlings’ development and improves the probability of survival of germinated seeds at the plant’s highest stage of vulnerability. According to all the above said, we have included a balanced supply of micronutrients in our formulation.

Products and Getting an Optimum Performance in Grains: Our PGPR products utilized according to the recommendations indicated in the packaging can achieve the following performance improvements in the field compared to a control without Biofertilizing (all graphic indicate kg/Ha):

PGPR Corn and Sorghum: Improves the Yield of 20.1% in Corn and of 58.7% in sorghum.

PGPR Wheat and Barley: Improves the yield of 12.3% in Wheat.

PGPR Sunflower: Improves the yield of 19.7 % in Sunflower.

PGPR Soya: Improves the yield of 11.7 %.

We are developing new products, which we hope will be in the marked as soon as possible.

The expected performance improvement for Green Max Soya Inoculant is:
-Green Max Soya: Improves in yield of 10.3%.

Expectations: Biotechnology advances at a national and international level allow us to discern a wide variety of new products which can be applied to the world’s most distributed cultivations, and at the same time they allow to continuously improve the products already available in the market.

This advance is strongly driven by the necessity to satisfy the growing demand for food by the world’s population. Besides, the impact of human activity is putting the environment at risk, due to this it is indispensable to incorporate maximum efficiency production technologies, which are sustainable along the time and environment friendly. This is the field we are determined to take, and this is the reason why we are constantly improving and thinking in widening our offer of products to the national and international market.

To facilitate the incorporation of these new technologies we have developed products with 2 and even 3 types of bacteria which are applied in a single step and with the necessary stability adequate to your cultivations’ requirements. We have been the first ones to develop and commercialize this sort of product and we are the most experienced in the market.

It is a product prepared with active Rhizobios (endosymbiotic bacteria of leguminous) so that they can colonize the roots at the moment of the application efficiently. Due to this fact the inoculant is the means in which these bacteria are transported from the elaboration up to the moment of the application. The inoculant with the longest history and best known in the market is the one including Rhizobio Bradyrhizobium japonicum, that is the guest of Glycine max (Soya).

It is a product that supplies active PGPR (growth promoters) which are able to increase your cultivation’s performance. The difference with Inoculants is that Biofertilizers have a wider range of application and do not generate differentiated structures when the symbiotic interaction happens. Nevertheless, the experience of Green Quality S.A. has allowed us to improve the formulations and the selection of stocks of the different products of the PGPR line to produce, in this way, the maximum performances in the cultivations where each product is applied.

Most of the PGPR are bacteria of free life which are beneficial for certain types of cultivations. Such is the case of Pseudomonas fluorescens and Azospirillum brasilense. There exist many others PGPR bacteria and microorganisms, but they have not such a wide acknowledged effect as vegetable growth promoters. We are working to incorporate other PGPR as promising as the ones already incorporated.

Rhizobios are also PGPR, to promote vegetable growth although in specific plants. Green Quality S.A. developed the first tri bacterial growth promoter specific for Soya that includes the Bradyrhizobium japonicum, Pseudomonas fluorescens and Azospirillum brasilense PGPR. This product combines both advantages of Inoculation and Biofertilization in Soya, allowing, once again, its application in a single step.

The initials PGPR come from English Plant Growth Promoting Rhizobacteria and refer to a group of soil bacteria which has the capacity of promoting plants’ growth. 

Under appropriate management conditions these bacteria increase cultivations’ productivity.

PGPR (growth promoters) are natural non pathogenic inhabitants of the soil which associate to the roots of numerous vegetable species, using substances excreted by the roots and in turn supplying the plants with substances that promote their growth. Besides they can protect a plant against the attack of pathogens, working in this way as biocontrollers. Another beneficial effect that produces better yields.

In spite of finding them freely in soils, they are only able to produce important agronomic benefits, increasing cultivations’ performance in values between 5 to 30% above non inoculated controls, when they are applied to seeds or roots under optimum conditions. These optimum conditions are: a) high numbers; b) Appropriate physiological conditions of bacterial growth; and c) specific stocks of phytoregulators.

Bradyrhizobium japonicum: It is the best known Rhizobios because it is the endosymbiont (symbiotic organism that lodges inside its host, in this case the Soya plant) which colonizes Soya plants’ roots forming Nodules where the Biological Fixation of Nitrogen (BFN) occurs.

When establishing an endosymbiotic relationship between the guest (Rhizobios) and the host (Leguminous plant), the host forms a characteristic structure (nodule) with optimum appropriate conditions so as the Rhizobios changes its metabolism and becomes what is known as Bacteroides.

The Bacteroides is a Rhizobios that dedicates the energetic resources supplied by the plant to produce the Biological Fixation of Nitrogen, which is an infrequent reaction in nature due to the difficulty of making this so little reactive gas into a form available to living beings. The plant makes this effort because it knows that in exchange it will obtain a resource which is normally infrequent in the soil.

Leguminous have high protein content in their grains, and due to this fact a high value in the market, just because they can store a big amount of nitrogen, which they obtain both from the soil and the air. This fact makes them very valuable ecologically as well as economically. Not to apply Green Quality Max to your Soya cultivation means not to take the chance to use to a maximum this unequalled advantage supplied by Glycine max, the Soya plant. If you only apply chemical fertilizers, you will never achieve the top performance or the quality this cultivation can produce. National studies suggest that BFN incorporates over 55% of the Nitrogen in Soya cultivation

To maximize the performance in Soya, and without losing the advantage of BFN, you can use Bradyrhizobium japonicum combined with growth promoting bacteriaAzospirillum brasilense and Pseudomonas fluorescens in the PGPR Soya ® product, which allows obtaining even better performance results due to the synergic effect of these beneficial bacteria specifically selected for this cultivation.

Azospirillum brasilense: It is the PGPR most used in Agriculture, and because of this fact, one of the most studied for its vegetable growth promoting effects. The results achieved all over the world placed it amongst the bacteria most used in commercial Biofertilizers, since 1970, when Dr. J. Dobereiner and his assistants rediscovered, in Brazil, this microorganism’s capacity to stimulate non leguminous cultivations’ growth. Their research allowed reconsidering the application of Biofertilizers in extensive cultivations, the same way that leguminous Inoculation was already used successfully. 

This bacterium has the capacity of fixing atmospheric nitrogen and converting it into ammonium, a form of N which can be used by the plant to synthesize amino acids and proteins.

Another very important characteristic is that this bacterium can associate to the roots’ surface in non leguminous plants such as cereal and grasses. 

Nevertheless its promoting effect is due mainly to other qualities of this microorganism. The most important one, to benefit plants’ growth, is the capacity to produce phytohormones. The most remarkable effect of this capacity is the stimulation of root development, which produces a bigger surface to attract nutrients and therefore a higher growth capacity. 

A. brasilense produces auxin, cytokinin, gibberellin phytohormones, ethylene and abscissae acid, depending upon the physiological conditions of the bacterial culture. All these substances act as regulators triggering development processes in the plants and also protecting them against stress conditions. 

A culture of A. brasilense under adequate physiologic conditions will provide the plant with an adequate proportion of phytohormones. Cytokinins induce cell division favouring germination among other processes. Auxins increase rooting capacity because they produce a higher number of lateral roots and root hairs. Gibberellins participate in the germination and the lengthening of the stem. Ethylene and abscissae acid are considered stress hormones and protect the plant under these conditions

Besides this bacterium has an ample metabolic versatility which allows it to live under a wide range of environmental conditions. Its Metabolism of C and N is flexible, adapting to stress and fasting conditions, using reserve substances which are previously stored (polyhydroxibutyrates). On the other hand it also produces siderophorus to attract iron from the soil, increasing the plant’s nutrition and diminishing the population of other pathogenic microorganisms from around the roots which compete for this nutrient.

Picture 1: Photograph of an oldened Azospirillum brasilense grown in Agar Red Congo. A red pigmentation, undulating hedges and wrinkles can be observed (Magnifying 10X).

-Capacity to fix atmospheric Nitrogen which makes it possible to diminish the dose of chemical fertilizers with the resulting reduction in costs and environment friendly practices.

- Capacity to promote development of the plant’s root system, facilitate the implanting and increase absorption of water and mineral nutrients.

 Protects the plant against short term hydric stress.

- Improves plant’s nutrition, nitrogenised as well as phosphatised.

- Diminishes phytopathogenic populations in roots, bacterial as well as fungical.

Pseudomonas fluorescens: Belongs to the PGPR group and the different stocks are abundant on the roots’ surface since they are versatile in their metabolism and are able to use several substrates produced by the plants, but do not establish a symbiotic relationship. J W Koepper and M N Schroth’s work together with their assistants’ work showed in U.S.A. that biocontrolling agents such as Pseudomonas fluorescens andPseudomonas putida (the latter one with a higher risk for human health) can act as pesticides and control diseases coming from the soil. Nevertheless, stocks selection allowed obtaining Pseudomonas which also supplies other growth promoting mechanisms.

Nowadays the main growth promoting characteristic of the stocks of commercializedPseudomonas fluorescens is their high phosphorus solubilizing capacity, making it available for the plant. This is an utterly important feature for soils having these nutrients in a mineral form because even if the nutrient is there, the plant can not incorporate it. This microorganism excretes some substances into the soil which make phosphorus soluble, allowing its bioavailability for the plant.

Some stocks can also synthesize phytohormones.

It acts as a biocontroller since it produces antagonist compounds of other pathogenic microorganisms of the soil, such as antibiotics of the type of phenacins and hydrocyanic acid and pigments as pio-verdancy which are fluorescent under UV light, and works as siderophorus taking iron from the surroundings, which prevents the growth of microorganisms sensitive to the lack of this nutrient.

Picture 2:

Pseudomona fluorescens grown in Nutritive Agar. Fluorescence under UV can be observed due to the production of the fluorescent pigment pio-verdancy.

How do PGPR promote cultivations’ growth?

Plants roods offer an ecologic niche for the proliferation of soil bacteria which live from substances exuded by the root. Theses substances are mainly carbon compounds useful as a source of energy for microorganisms. This is why the area surrounding the plants’ roots has an unusual microbial activity, compared to the common soil. This notorious difference produced the term “rhizosphere”, which refers to the soil zone surrounding the plants’ roots. In the rhizosphere, either beneficial, or innocuous or harmful organisms may proliferate.The advantage of PGPR (growth promoters) is based in the fact that these bacteria utilize carbon compounds, which the plant has in excess due to the photosynthesis, to perform some processes which have beneficial effects for the plant as much as for the bacterium. Plants have developed some mechanisms to allow some degree o selection on the microorganisms which settle in the source of carbon compounds, that is: the root surface and the surrounding zone. But when the plant does not find adequate microorganisms can not prevent other ones from colonizing this region, increasing the risk of finding harmful organisms and missing the possibility of obtaining a benefit in exchange for its exuded products. This is why applying Inoculants or Biofertilizers at early stages is so advantageous, since you can make it sure that the plant can develop a beneficial interaction in the root, which is the most vulnerable region.In turn PGPR (growth promoters) supply the plant with metabolites which can act as growth regulating signal molecules. 
Amongst these compounds we can find not only vegetable hormones which enhance root development but also vitamins and nitrogenised compounds. In this way bacteria affect directly plants’ growth through the production of phytohormonal substances such as auxins, gibberellins and cytokinins. But these bacteria also favour, in an indirect way, plants’ growth because they have the capacity to secrete compounds with antimicrobial and antifungal activity as well as siderophorus to increase iron absorption in the soil, diminishing in this way the phytopathogen population.

Plants absorb phosphorus almost exclusively in the inorganic form which can be found in the soil solution. In this way, even in soils with a good content of total phosphorus, cultivations’ demand during the growing period exceeds the availability of this nutrient. The phosphorus of the soluble fraction must be restored constantly from easy-to-extract forms, organic as well as inorganic ones. The availability of this nutrient depends, in great part, on microbial action. 

The high capacity some microorganisms have to make phosphorus soluble, as for example Pseudomonas fluorescens, is due to two features:

a) They produce and release organic acids into the surroundings (citric acid, oxalic acid, glycolic acid) which diminish soil pH favouring, in this way, inorganic phosphorus solubilization and releasing the phosphate into the soil solution. 

b) The other way is through the phophatases they contain, that are hydrolytic mono and di-esterases enzymes which free the phosphate groups from the organic matter and they go into the soil solution. 

Both ways generate a higher amount of phosphate to be absorbed by the plants’ roots.

Why Inoculate your seeds with PGPR (growth promoters)?

Our intention with this brief description of the mechanisms involved in the improvement of your cultivation’s performance is that the final consumer of the Inoculants and Biofertilizers technologies understands the advantages of PGPR products and dares to incorporate them with confidence. But his confidence should not be based on these explanations we have offered but on his own experience with these products. That is why our enterprise is utterly interested that producers themselves have the last word and evaluate the advantages they obtain.

We know that the only way for a producer to prove the efficiency of PGPR products is applying the product to a part of his cultivation and compare it to the part without application, being the most important result the difference in yield of grains per hectare. Those who are already convinced of the benefit obtained do not leave parts of their cultivation without Biofertilizing or Innoculating, and we hope that those who still disbelieve the potentialities will feel encouraged to carry out some tests of our products in a lesser scale and observe not only the advantages in performance but also the advantages related to the cultivations’ sanity, root development, implanting percentage, falling index number, amongst others, along all the cycle.